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lentiviral plko 1 shrna construct shgfp  (Addgene inc)


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    Addgene inc lentiviral plko 1 shrna construct shgfp
    Lentiviral Plko 1 Shrna Construct Shgfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shgfp/pLKO%2E1+puro+GFP+siRNA+(Plasmid+%2312273)/10__1158_slash_0008___5472__can___26___1063-43-1-6
    Average 94 stars, based on 30 article reviews
    lentiviral plko 1 shrna construct shgfp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Oncogenic enhancers prime quiescent metastatic cells to escape NK immune surveillance by eliciting transcriptional memory.
    Article Snippet: .. Cells expressing shRNAs against SOX9 or GFP were obtained transducing MD cells with the lentiviral vector pLKO.1 shSOX9 (Addgene #40644) or shGFP (Addgene #110419) at a multiplicity of infection (MOI) of 10. .. Cells expressing inducible tetO SOX9 were obtained transducing tIMECwith the lentiviral vector FUW tetOSOX9 (Addgene#41080) at a multiplicity of infection (MOI) of 1.

    Infection:

    Article Title: Oncogenic enhancers prime quiescent metastatic cells to escape NK immune surveillance by eliciting transcriptional memory.
    Article Snippet: .. Cells expressing shRNAs against SOX9 or GFP were obtained transducing MD cells with the lentiviral vector pLKO.1 shSOX9 (Addgene #40644) or shGFP (Addgene #110419) at a multiplicity of infection (MOI) of 10. .. Cells expressing inducible tetO SOX9 were obtained transducing tIMECwith the lentiviral vector FUW tetOSOX9 (Addgene#41080) at a multiplicity of infection (MOI) of 1.

    Article Title: Breast cancer cell resistance to hormonal and targeted therapeutics is correlated with the inactivation of the NR6A1 axis.
    Article Snippet: .. To exclude the off-target effects of NR6A1 shRNAs, we used the parallel infection with virion particles formed by the same lentiviral vector-coding shGFP (pLKO.1 GFP shRNA #30323, Addgene, target sequence: 5’GCAAGCTGACCCTGAAGTTCAT3’), which is absent in the human genome. .. The viral particles contained in the conditioned medium were collected 24 and 48 h after transfection and then added to MCF-7 cells in the presence of 8 μg/mL Polybrene (Sigma, USA).

    Control:

    Article Title: Fatty acid oxidation regulates cellular senescence by modulating the autophagy-SIRT1 axis
    Article Snippet: .. The sequences for shRNAs are as follows. shCPT1A #1 5’-CCGGGGATGGGTATGGTCAAGATCTCTCGAGAGATCTTGACCATACCCATCCTTTTT-3’; shCPT1A #2 5’-CCGGGGTGGTTTGACAAGTCGTTCACTCGAGTGAACGACTTGTCAAACCACCTTTTT-3’. shGFP used as a control was purchased form Addgene (#30323). ..

    Article Title: Fatty acid oxidation regulates cellular senescence by modulating the autophagy-SIRT1 axis
    Article Snippet: .. The sequences for shRNAs are as follows. shCPT1A #1 5’-CCGGGGA TGGGTATGGTCAAGATCTCTCGAGAGATCTTGACCATACC CATCCTTTTT-3’; shCPT1A #2 5’-CCGGGGTGGTTTGACAAGTC GTTCACTCGAGTGAACGACTTGTCAAACCACCTTTTT-3’. shGFP used as a control was purchased form Addgene (#30323). ..

    Article Title: The BET Inhibitor JQ1 Potentiates the Anticlonogenic Effect of Radiation in Pancreatic Cancer Cells
    Article Snippet: .. Briefly, Panc1 cells were transfected using PEI (Polysciences Inc., Warrington, VA, USA) and Mission shRNA (Millipore Sigma) targeting BRD2 or BRD4. shGFP served as vector control (Addgene, Watertown, MA, USA). .. Selection of stable transfectants was carried out using 10 μg/ml puromycin (ENZO Life Sciences, Farmingdale, NY, USA).

    Plasmid Preparation:

    Article Title: Breast cancer cell resistance to hormonal and targeted therapeutics is correlated with the inactivation of the NR6A1 axis.
    Article Snippet: .. To exclude the off-target effects of NR6A1 shRNAs, we used the parallel infection with virion particles formed by the same lentiviral vector-coding shGFP (pLKO.1 GFP shRNA #30323, Addgene, target sequence: 5’GCAAGCTGACCCTGAAGTTCAT3’), which is absent in the human genome. .. The viral particles contained in the conditioned medium were collected 24 and 48 h after transfection and then added to MCF-7 cells in the presence of 8 μg/mL Polybrene (Sigma, USA).

    Article Title: VIRMA-mediated m 6 A modification regulates forebrain formation through modulating ribosome biogenesis
    Article Snippet: The AAV-shRNA vector (AAV: ITR-CMV-Cerulean-SV40-shRNA-U6-ITR), as described in a previous study , was generously provided by J. Xia from Hong Kong University of Science and Technology (Guangzhou). .. The shCON used here was shGFP as in Addgene plasmid no. 30323. .. All constructs were confirmed by Beijing Genomics Institute (BGI) sequencing.

    shRNA:

    Article Title: Breast cancer cell resistance to hormonal and targeted therapeutics is correlated with the inactivation of the NR6A1 axis.
    Article Snippet: .. To exclude the off-target effects of NR6A1 shRNAs, we used the parallel infection with virion particles formed by the same lentiviral vector-coding shGFP (pLKO.1 GFP shRNA #30323, Addgene, target sequence: 5’GCAAGCTGACCCTGAAGTTCAT3’), which is absent in the human genome. .. The viral particles contained in the conditioned medium were collected 24 and 48 h after transfection and then added to MCF-7 cells in the presence of 8 μg/mL Polybrene (Sigma, USA).

    Article Title: The BET Inhibitor JQ1 Potentiates the Anticlonogenic Effect of Radiation in Pancreatic Cancer Cells
    Article Snippet: .. Briefly, Panc1 cells were transfected using PEI (Polysciences Inc., Warrington, VA, USA) and Mission shRNA (Millipore Sigma) targeting BRD2 or BRD4. shGFP served as vector control (Addgene, Watertown, MA, USA). .. Selection of stable transfectants was carried out using 10 μg/ml puromycin (ENZO Life Sciences, Farmingdale, NY, USA).

    Sequencing:

    Article Title: Breast cancer cell resistance to hormonal and targeted therapeutics is correlated with the inactivation of the NR6A1 axis.
    Article Snippet: .. To exclude the off-target effects of NR6A1 shRNAs, we used the parallel infection with virion particles formed by the same lentiviral vector-coding shGFP (pLKO.1 GFP shRNA #30323, Addgene, target sequence: 5’GCAAGCTGACCCTGAAGTTCAT3’), which is absent in the human genome. .. The viral particles contained in the conditioned medium were collected 24 and 48 h after transfection and then added to MCF-7 cells in the presence of 8 μg/mL Polybrene (Sigma, USA).

    Transfection:

    Article Title: The BET Inhibitor JQ1 Potentiates the Anticlonogenic Effect of Radiation in Pancreatic Cancer Cells
    Article Snippet: .. Briefly, Panc1 cells were transfected using PEI (Polysciences Inc., Warrington, VA, USA) and Mission shRNA (Millipore Sigma) targeting BRD2 or BRD4. shGFP served as vector control (Addgene, Watertown, MA, USA). .. Selection of stable transfectants was carried out using 10 μg/ml puromycin (ENZO Life Sciences, Farmingdale, NY, USA).



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